Microbial amplicon sequencing dataset for glacial ice and snow on the Tibetan Plateau (V1.0) (2016-2020)

The dataset contains microbial amplicon sequencing data from a total of 269 ice samples collected from 15 glaciers on the Tibetan Plateau from November 2016 to August 2020, including 24K Glacier (24K), Dongkemadi Glacier (DKMD), Dunde Glacier (DD), Jiemayangzong Glacier (JMYZ), Kuoqionggangri Glacier (KQGR), Laigu Glacier (LG), Palung 4 Glacier (PL4), Qiangtang 1 Glacier (QT), Qiangyong Glacier (QY), Quma Glacier (QM), Tanggula Glacier (TGL), Xiagangjiang Glacier (XGJ), Yala Glacier (YA), Zepugou Glacier (ZPG), ZhufengDongrongbu Glacier (ZF). The sampling areas ranged in latitude and longitude from 28.020°N to 38.100°N and 86.28°E to 95.651°E. The 16s rRNA gene was amplified by polymerase chain reaction (PCR) using 515F/907R (or 515F/806R) primers and sequenced with the Illumina Hiseq2500 sequencing platform to obtain raw data. The selected primer sequences were "515F_GTGYCAGCMGCCGCGGTAA; 907R_CCGTCAATTCMTTTRAGTTT" "515F_GTGCCAGCMGCCGCGG; 806R_ GGACTACHVGGGTWTCTAAT". The uploaded data include: sample number, sample description, sampling time, latitude and longitude coordinates, sample type, sequencing target, sequencing fragment, sequencing primer, sequencing platform, data format and other basic information. The sequencing data are stored in sequence file data format forward *.1.fq.gz and reverse *.2.fq.gz compressed files.

0 2022-07-22

Genome re sequencing data of main domestic animals (chickens) (2020-2022)

In order to describe the distribution pattern of genetic diversity of main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third pole area), and clarify its related genetic background. In 2020, we extracted the total DNA from 266 global chicken blood, tissue and other DNA tissue samples, built a database and sequenced the whole genome. At the same time, we downloaded the published chicken genome data, and carried out population analysis of 863 chicken genomes, so as to provide basic data for exploring the historical events of domestication, migration and expansion of domestic chickens in the pan third pole region, and further explore the adaptation mechanism of domesticated animals to harsh environments such as drying. Articles related to this data set have been published. All data in this data set can be downloaded online from fastq, BAM, VCF and SNP files.

0 2022-06-27

Plant de novo genome sequencing data the germplasm (2021)

We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.

0 2022-06-23

Plant Hi-C sequencing data of the germplasm (2021)

We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.

0 2022-06-23

Plant genome resequencing data of the germplasm (2021)

We investigated and collected the germplasm resources of cyanine in the Qinghai Tibet Plateau and its surrounding areas, carried out homogenous garden experiments to obtain phenotypic data, used genome sequencing technology to obtain data libraries and construct high-quality reference genomes. Using the re sequencing technology to analyze the structure of the cyanine population, combined with the early human migration and diffusion routes, this paper explores the historical process of the formation of the modern geographical distribution pattern of the cyanine on the Qinghai Tibet Plateau. By correlation analysis with phenotypic data, the adaptive mechanism of modern populations of cyanine was analyzed. Understand the environmental differences of the pan third pole and the impact of human activities and cultural differences in different regions on the migration, adaptation and domestication of plants on the Qinghai Tibet Plateau from the whole genome level.

0 2022-06-23

Photos and videos of the Second Tibetan Plateau Scientific Expedition and Research on conservation and sustainable utilization of microbial diversity in the plateau (2020-2021)

The Second Tibetan Plateau Scientific Expedition and Research Task V Theme III "Conservation and Sustainable Utilization of Plateau Microbial Diversity" (2019QZKK0503) carried out more than 30 field scientific expeditions in the first and second years. Footprints cover most of the Tibetan Plateau, including the investigation of glaciers (such as Qiangyong Glacier, Tanggula Glacier, Everest East Rongbu glacier, Jiemayangzong Glacier, Palung 4 Glacier, etc.), lakes, soils, fungi, lichens, animals in Southeast Tibet, Qiangtang Plateau, Cocosili and Himalayan region. The dataset contains 6,471 photos and videos, including habitat photos, working photos, and scientific images collected during the first and second years of fieldwork.

0 2022-06-15

Transcriptome data of plain population before entering Tibet (2019)

People in the plain often have altitude reaction after entering Tibet. In order to deeply analyze the change pattern of genomic expression profile in the process of altitude response and altitude acclimation. Based on the mRNA transcriptome sequencing method, we have obtained the transcriptome sequencing data of 46 individuals before entering Tibet (collection place: Chongqing). We first collected the peripheral blood samples of 46 plain Han individuals, treated the peripheral blood with red blood cell lysate (Tiangen), centrifuged at 4000 rpm for 10 min, separated and extracted white blood cells, and extracted the total RNA of each sample by Trizol method. Then 46 libraries were constructed by poly (a) capture method. Poly (a) + mRNA library was isolated from 1 g total RNA of each sample with oligo (DT) beads. The construction of RNA SEQ library was carried out according to the preparation scheme of truseq RNA library. The 46 RNA libraries were double ended sequenced using novaseq platform. The sequencing results were 150 BP reads fastq files, and the data volume of each sample exceeded 6.0 GBP. The transcriptome data of plain people before entering Tibet can be used as the baseline data after entering Tibet. By comparing and analyzing the transcriptome data of plain people before and after entering Tibet, screening the significantly differentially expressed genes before and after entering Tibet, and annotating the biological functions of differentially expressed genes, we can deeply analyze the gene expression change mode and function regulation network mechanism in the process of altitude reaction and altitude acclimatization.

0 2022-06-03

Qinghai Tibetan sheep and Qinghai fine wool sheep tissue sample data set (2021)

In order to collect the special germplasm resources of Qinghai Tibet Plateau and excavate the molecular markers affecting the special germplasm resources, individual sheep with excellent ectopic spots were selected for marker assisted selection, propagation and generation breeding according to the genetic marker information, so as to cultivate the families of special germplasm resources. In 2021, this sub project (2019QZKK05010704) widely collected samples of Qinghai Tibetan sheep and Qinghai fine wool sheep in Haibei, Qinghai, and continued to establish and expand the first and second core groups in Ledu agricultural experimental station and Qinghai Sanjiaocheng sheep breeding farm. This data set contains the basic information of 1050 tissue samples, including variety, collection place, collection time, gender, tissue type, preservation method, etc.

0 2022-05-19

Genomic genetic characteristics and germplasm resources data set of Hevea cattle in southern Tibet and its surrounding areas (2021)

In order to analyze the genomic characteristics, domestication, population history, genetic diversity and population differentiation characteristics of large forehead cattle, as well as the genetic basis of excellent meat economic traits of large forehead cattle, this sub project (2019QZKK05010703) investigated the genetic resources of Dulong cattle and Gaofeng cattle in Kunming and Wenshan Miao Autonomous Prefecture of Yunnan Province in 2021, and collected tissue samples of heart, liver, spleen, lung and kidney. Provide scientific theoretical reference for the protection, development and utilization of large cattle germplasm resources, and promote the development of high-end beef cattle industry in China. This data set contains individual photos and tissue sample information table, which records basic information such as variety, collection place, collection time, sample type, sampling position and so on.

0 2022-05-19

Sichuan small animal specimens and tissue samples, Tibet infrared camera photo data set (2021)

In order to analyze the animal diversity pattern of the Qinghai Tibet Plateau and establish the corresponding animal specimen database. In 2021, this sub project (2019QZKK05010109) focused on Liangshan Yi Autonomous Prefecture and Ganzi Tibetan Autonomous Prefecture. A total of 271 specimens of local wild animals, such as Apodemus agrarius and social rats, were collected. The solid samples include animal individuals, skins, tissues, etc. This data set contains one specimen information table (271 copies), one tissue sample information table (271 copies), and photos taken by Tibet Chayu moto infrared camera. The sample information table contains basic sample information such as species, variety, detailed sampling place, sample type, collection time, collector and storage method, which is stored in the form of Excel. Photos, stored in JPG format.

0 2022-05-18